Journal: Toxics

Article Title: Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

doi: 10.3390/toxics9110289

Figure Lengend Snippet: Bax/Bcl-2 ratio ( A ) and caspases levels ( B ) (mean ± SD) in liver and gills of Danio rerio ( n = 3 pools of 10 individuals each pool) and Perca fluviatilis ( n = 6). Tissue extracts from all groups were immunoblotted for Bax, Bcl-2 and caspases. Blots and dots were quantified using scanning densitometry. Representative blots and dots are shown. Mann–Whitney U test was employed to test for significance at p < 0.05 between all experimental groups. * denotes significant differences ( p < 0.05) compared to the control group ( n = 3 pools of 10 individuals and n = 6 for D. rerio and P. fluviatilis respectively).

Article Snippet: The blots were detected using enhanced chemiluminescence (Chemicon) on Fuji Medical X-ray film and quantified by densitometry scanning laser (GelPro Analyzer Software, GraphPad, San Diego, CA, USA).

Techniques: MANN-WHITNEY, Control

Journal: Toxics

Article Title: Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics

doi: 10.3390/toxics9110289

Figure Lengend Snippet: LC3II/I ratio ( A ) and SQSTM1/p62 levels ( B ) (mean ± SD) in liver and gills of Danio rerio ( n = 3 pools of 10 individuals) and Perca fluviatilis ( n = 6). Tissue extracts from all groups were immunoblotted for LC3II/I and SQSTM1/p62. Blots and dots were quantified using scanning densitometry. Representative blots and dots are shown. Mann–Whitney U test was employed to test for significance at p < 0.05 between all experimental groups. * denotes significant differences ( p < 0.05) compared to the control group ( n = 3 pools of 10 individuals and n = 6 for D. rerio and P. fluviatilis respectively), while z and p denote significant differences ( p < 0.05) between D. rerio and P. fluviatilis respectively.

Article Snippet: The blots were detected using enhanced chemiluminescence (Chemicon) on Fuji Medical X-ray film and quantified by densitometry scanning laser (GelPro Analyzer Software, GraphPad, San Diego, CA, USA).

Techniques: MANN-WHITNEY, Control

Journal: Journal of neuroendocrinology

Article Title: Lack of Annexin 1 Results in an Increase in Corticotroph Number in Male but not Female Mice

doi: 10.1111/j.1365-2826.2006.01481.x

Figure Lengend Snippet: Western blot analysis of interleukin (IL)-6 in anterior pituitary from ANXA1 wild-type (WT) and null mice. (a) IL-6 and (c) β-actin Western blots: wild-type male, lanes 1 and 2; ANXA1 null male, lanes 3 and 4; (b) IL-6 and (D) β-actin Western blots: wild-type female, lanes 1 and 2; ANXA1 null female, lanes 3 and 4. Total integrated densitometry data for male tissue (e) and female tissue (f). Data are mean ± SEM (n = 5 experiments) and represent integrated densities for IL-6 expressed as percentage wild-type; *P < 0.05, **P < 0.01 versus wild-type of the same gender.

Article Snippet: PCR products were separated on a 1% agarose gel stained with ethidium bromide, photographed and analysed by scanning densitometry (TINA software version 2.10, Raytest Isotopenmessgeraete GmbH, Straubenhardt, Germany).

Techniques: Western Blot